Profacgen Enhances RNA-Protein Interaction Analysis with Updated CLIP-Seq Service

Profacgen, a leading provider of cutting-edge genomic services, has recently updated its CLIP-Seq service offering that leverages the latest irCLIP-Seq technology, providing researchers with a powerful tool for investigating RNA-protein interactions and post-transcriptional regulatory networks.

RNA-binding proteins (RBPs) play a crucial role in post-transcriptional gene regulation, allowing cells to adapt to changing environments. CLIP-Seq (cross-linking immunoprecipitation sequencing) is a cutting-edge technique for mapping the RNA-binding landscape of RBPs on a genome-wide scale. Profacgen's updated CLIP-Seq service utilizes infrared fluorescent dyes instead of traditional radioactive materials, expanding the application potential of this technology. The service also employs thermostable group II intron reverse transcriptase for cDNA synthesis, streamlining the experimental procedure and improving efficiency.

Profacgen's CLIP-Seq service supports researchers in designing, developing, optimizing, and troubleshooting all stages of their CLIP projects. The application of CLIP-Seq is vast, enabling the analysis of RNA-protein interaction mechanisms, the functional mechanisms of lncRNAs/circRNAs, and the identification of potential miRNA targets. By providing insights into post-transcriptional regulatory networks, CLIP-Seq can facilitate a deeper understanding of gene expression and its role in various biological processes and diseases.

To facilitate the CLIP-Seq process, Profacgen recommends submitting UV cross-linked cell samples (ideally 10^7 cells) for their stability. Live cells in culture medium are also acceptable. The company conducts single-end 50-cycle sequencing with 20-40 million reads. Profacgen's comprehensive data analysis includes quality control, sequence alignment, DNA element analysis, peak annotation, gene ontology annotation, and KEGG pathway analysis. Researchers gain a thorough understanding of their data through this in-depth analysis.

Researchers can contact Profacgen's experts to design an optimal CLIP-Seq solution for their projects and receive support throughout the entire process. With its updated CLIP-Seq service, Profacgen continues to empower cutting-edge research in RNA-protein interactions and post-transcriptional regulation. For more information, please visit https://www.profacgen.com/clip-seq-service.htm.

About Profacgen

Profacgen is a leading provider of protein-related services, specializing in RNA-protein interaction analysis. The company's mission is to facilitate cutting-edge research in post-transcriptional regulation and gene expression. With its updated CLIP-Seq service, Profacgen reaffirms its commitment to providing researchers with the tools they need to advance the understanding of gene expression and its role in shaping biological systems.